Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (36)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Mitra, D. K.
Right arrow Articles by Roederer, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mitra, D. K.
Right arrow Articles by Roederer, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 11, No. 11, 1801-1810, November 1999
© 1999 Japanese Society for Immunology

Differential representations of memory T cell subsets are characteristic of polarized immunity in leprosy and atopic diseases

Dipendra K. Mitra1,3, Stephen C. De Rosa1, Andrew Luke2, Arumugam Balamurugan3, Binod K. Khaitan4, James Tung1, Narinder K. Mehra3, Abba I. Terr2, Anne O'Garra5, Leonore A. Herzenberg1, Leonard A. Herzenberg1 and Mario Roederer6

1 Department of Genetics and
2 Allergy Clinic, Immunology Division, Department of Medicine, Stanford University, Stanford, CA 94305, USA
3 Histocompatibility & Immunogenetics Department and
4 Department of Dermatology, All India Institute of Medical Sciences, New Delhi 110029, India
5 Department of Immunobiology, DNAX Research Institute, Palo Alto, CA 94304, USA
6 Department of Stomatology, San Francisco, CA 94143-0422, USA

Correspondence to: M. Roederer, Department of Stomatology, 521 Parnassus Ave C634C, San Francisco, CA 94143-0422, USA

We identified functionally polarized subsets of CD4 memory T cells on the basis of the expression of CD11a, CD45RA and CD62L. Within the several phenotypically distinct subsets of CD4 memory cells are two that, upon stimulation, produce primarily IL-4 (MT2, CD45RA–CD62L+CD11adim) or primarily IFN-{gamma} (MT1, CD45RA–CD62L–CD11abright). In addition, four other phenotypically distinct subsets of CD4 cells have unique cytokine profiles. To determine the clinical relevance of the representation of these cell types, we analyzed blood from patients with the chronic diseases leprosy and atopy. These diseases are characterized as immunologically polarized, since T cell responses in affected individuals are often strongly biased towards Th1 (dominated by IFN-{gamma} production) or Th2 (IL-4 production). We show here that this polarization reflects homeostatic or differentiation mechanisms affecting the representation of the functionally distinct subsets of memory CD4 T cells, MT1 and MT2. Significantly, the representation of the MT1 and MT2 subsets differs dramatically between subjects with tuberculoid leprosy (a Th1 disease), or lepromatous leprosy or atopic disease (Th2 diseases). However, there was no difference in the cytokine profiles of these or any of the other finely resolved CD4 subsets, when compared between individuals across all disease states. Thus, it is the representation of these subsets in peripheral blood that is diagnostic of the polarized state of the immune system.

Keywords: cytokine profile, flow cytometry, intracellular cytokine staining, Th1, Th2

Transmitting editor: R. Hardy


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
P. K. Sharma, P. K. Saha, A. Singh, S. K. Sharma, B. Ghosh, and D. K. Mitra
FoxP3+ Regulatory T Cells Suppress Effector T-Cell Function at Pathologic Site in Miliary Tuberculosis
Am. J. Respir. Crit. Care Med., June 1, 2009; 179(11): 1061 - 1070.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. Lundgren, C. Trollmo, A. Edebo, A.-M. Svennerholm, and B. S. Lundin
Helicobacter pylori-Specific CD4+ T Cells Home to and Accumulate in the Human Helicobacter pylori-Infected Gastric Mucosa
Infect. Immun., September 1, 2005; 73(9): 5612 - 5619.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Song, R. L. Rabin, B. J. Hill, S. C. De Rosa, S. P. Perfetto, H. H. Zhang, J. F. Foley, J. S. Reiner, J. Liu, J. J. Mattapallil, et al.
Characterization of subsets of CD4+ memory T cells reveals early branched pathways of T cell differentiation in humans
PNAS, May 31, 2005; 102(22): 7916 - 7921.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. Lundgren, E. Stromberg, A. Sjoling, C. Lindholm, K. Enarsson, A. Edebo, E. Johnsson, E. Suri-Payer, P. Larsson, A. Rudin, et al.
Mucosal FOXP3-Expressing CD4+ CD25high Regulatory T Cells in Helicobacter pylori-Infected Patients
Infect. Immun., January 1, 2005; 73(1): 523 - 531.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. Kruse, K. Varming, and O.B. Christiansen
Prospective, serial investigations of in-vitro lymphocyte cytokine production, CD62L expression and proliferative response to microbial antigens in women with recurrent miscarriage
Hum. Reprod., November 1, 2003; 18(11): 2465 - 2472.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. Lundgren, E. Suri-Payer, K. Enarsson, A.-M. Svennerholm, and B. S. Lundin
Helicobacterpylori-Specific CD4+ CD25high Regulatory T Cells Suppress Memory T-Cell Responses to H. pylori in Infected Individuals
Infect. Immun., April 1, 2003; 71(4): 1755 - 1762.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Sahaf, K. Heydari, L. A. Herzenberg, and L. A. Herzenberg
Lymphocyte surface thiol levels
PNAS, April 1, 2003; 100(7): 4001 - 4005.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Schwaiger, A. M. Wolf, P. Robatscher, B. Jenewein, and B. Grubeck-Loebenstein
IL-4-Producing CD8+ T Cells with a CD62L++(bright) Phenotype Accumulate in a Subgroup of Older Adults and Are Associated with the Maintenance of Intact Humoral Immunity in Old Age
J. Immunol., January 1, 2003; 170(1): 613 - 619.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
B. S. Lundin, C. Johansson, and A.-M. Svennerholm
Oral Immunization with a Salmonella enterica Serovar Typhi Vaccine Induces Specific Circulating Mucosa-Homing CD4+ and CD8+ T Cells in Humans
Infect. Immun., October 1, 2002; 70(10): 5622 - 5627.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
L. A. Herzenberg, D. Parks, B. Sahaf, O. Perez, M. Roederer, and L. A. Herzenberg
The History and Future of the Fluorescence Activated Cell Sorter and Flow Cytometry: A View from Stanford
Clin. Chem., October 1, 2002; 48(10): 1819 - 1827.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. R. Mascola, M. K. Louder, C. Winter, R. Prabhakara, S. C. De Rosa, D. C. Douek, B. J. Hill, D. Gabuzda, and M. Roederer
Human Immunodeficiency Virus Type 1 Neutralization Measured by Flow Cytometric Quantitation of Single-Round Infection of Primary Human T Cells
J. Virol., April 16, 2002; 76(10): 4810 - 4821.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
S. J. M. ten Bruggencate, L. M. Hillyer, and B. D. Woodward
The Proportion of CD45RA+CD62L+ (Quiescent-Phenotype) T Cells within the CD8+ Subset Increases in Advanced Weight Loss in the Protein- or Energy-Deficient Weanling Mouse
J. Nutr., December 1, 2001; 131(12): 3266 - 3269.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
S. C. De Rosa, D. K. Mitra, N. Watanabe, L. A. Herzenberg, L. A. Herzenberg, and M. Roederer
V{delta}1 and V{delta}2 {gamma}{delta} T cells express distinct surface markers and might be developmentally distinct lineages
J. Leukoc. Biol., October 1, 2001; 70(4): 518 - 526.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.