International Immunology, Vol. 11, No. 11, 1731-1738,
November 1999
© 1999 Japanese Society for Immunology
Antigen-induced TCR–CD3 down-modulation does not require CD3
or CD3
cytoplasmic domains, necessary in response to anti-CD3 antibody
Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Case 906, 13288 Marseilles, Cedex 9, France
Correspondence to: C. Boyer
We studied cytotoxic T lymphocyte (CTL) clones expressing cytoplasmic domain-deleted CD3
and CD3
chains. These cells retained efficient antigen-specific cytolysis. Because the cytoplasmic domains of native CD3 and CD3 chains contain a dileucine-based and a tyrosine-based motif thought to be important for receptor endocytosis, we compared TCR–CD3 down-modulation on the CTL clones expressing or not these domains. We found that antigen-induced TCR–CD3 down-modulation was not dependent on either the CD3 or CD3 cytoplasmic domains. This contrasts with phorbol ester- and anti-CD3 mAb (soluble or plastic-coated)-induced TCR–CD3 down-modulation, that are respectively dependent on CD3 and on either CD3 or CD3 cytoplasmic domains, suggesting that differences may exist between the mechanisms of TCR–CD3 down-modulation in response to the three stimuli. TCR–CD3 down-modulation in response to antigen was demonstrated by confocal microscopy to be associated with TCRß chain internalization, whether CD3 and CD3 were native or truncated. Inhibition by the protein tyrosine kinase inhibitor PP1 of TCR–CD3 down-modulation in response to antigen was also similar whether CD3 and CD3 cytoplasmic domains were present or not. These properties of receptor down-modulation are discussed with respect to the requirements for TCR engagement on antigen-presenting cells.
Keywords: cell–cell interactions, cytotoxic T lymphocyte, protein kinases, rodent, TCR
Transmitting editor: M. M. Davis
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Davanture, J. Leignadier, P. Milani, P. Soubeyran, B. Malissen, M. Malissen, A.-M. Schmitt-Verhulst, and C. Boyer Selective Defect in Antigen-Induced TCR Internalization at the Immune Synapse of CD8 T Cells Bearing the ZAP-70(Y292F) Mutation J. Immunol., September 1, 2005; 175(5): 3140 - 3149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Monjas, A. Alcover, and B. Alarcon Engaged and Bystander T Cell Receptors Are Down-modulated by Different Endocytotic Pathways J. Biol. Chem., December 31, 2004; 279(53): 55376 - 55384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. La Gruta, H. Liu, S. Dilioglou, M. Rhodes, D. L. Wiest, and D. A. A. Vignali Architectural Changes in the TCR:CD3 Complex Induced by MHC:Peptide Ligation J. Immunol., March 15, 2004; 172(6): 3662 - 3669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Torres, A. Alcover, D. A. Zapata, J. Arnaud, A. Pacheco, J. M. Martin-Fernandez, E. M. Villasevil, O. Sanal, and J. R. Regueiro TCR Dynamics in Human Mature T Lymphocytes Lacking CD3{gamma} J. Immunol., June 15, 2003; 170(12): 5947 - 5955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Dietrich, C. Menne, J. P. H. Lauritsen, M. von Essen, A. B. Rasmussen, N. Odum, and C. Geisler Ligand-Induced TCR Down-Regulation Is Not Dependent on Constitutive TCR Cycling J. Immunol., June 1, 2002; 168(11): 5434 - 5440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. von Essen, C. Menne, B. L. Nielsen, J. P. H. Lauritsen, J. Dietrich, P. S. Andersen, K. Karjalainen, N. Odum, and C. Geisler The CD3{gamma} Leucine-Based Receptor-Sorting Motif Is Required for Efficient Ligand-Mediated TCR Down-Regulation J. Immunol., May 1, 2002; 168(9): 4519 - 4523. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Magnan, V. Di Bartolo, A.-M. Mura, C. Boyer, M. Richelme, Y.-L. Lin, A. Roure, A. Gillet, C. Arrieumerlou, O. Acuto, et al. T Cell Development and T Cell Responses in Mice with Mutations Affecting Tyrosines 292 or 315 of the Zap-70 Protein Tyrosine Kinase J. Exp. Med., August 20, 2001; 194(4): 491 - 506. [Abstract] [Full Text] [PDF]
|
|