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International Immunology, Vol. 11, No. 1, 21-28, January 1999
© 1999 Japanese Society for Immunology

Characterization of CD4+CD8{alpha}{alpha}+ and CD4–CD8{alpha}{alpha}+ intestinal intraepithelial lymphocytes in rats

Katsuo Yamada1,2, Yuki Kimura1, Hitoshi Nishimura1, Yasushi Namii1,3, Mitsuya Murase2 and Yasunobu Yoshikai1

1 Laboratory of Host Defense and Germfree Life, Research Institute for Disease Mechanism and Control,
2 Department of Thoracic Surgery, and
3 Second Department of Surgery, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan

Correspondence to: Y. Yoshikai

Intestinal intraepithelial lymphocytes (i-IEL) of aged rats comprise CD4+CD8{alpha}{alpha}+ and CD4–CD8{alpha}{alpha}+ T cells expressing TCR {alpha}ß. In the present study, we compared characteristics between CD4+CD8{alpha}{alpha}+ and CD4–CD8{alpha}{alpha}+ i-IEL, which were purified by a cell sorter from the i-IEL of 6-month-old Lewis rats. Most of the CD4+CD8{alpha}{alpha}+ i-IEL were of the CD44high phenotype, while CD4–CD8{alpha}{alpha}+ i-IEL were CD44low. Vß usage in the CD4–CD8{alpha}{alpha}+ i-IEL was much diversified, while CD4+CD8{alpha}{alpha}+ i-IEL showed a skewed Vß repertoire. The CD4+CD8{alpha}{alpha}+ i-IEL but not the CD4–CD8{alpha}{alpha}+ i-IEL proliferated in response to syngeneic spleen cells, which was partially inhibited by addition of anti-MHC class I mAb. The CD4+CD8{alpha}{alpha}+ i-IEL produced IFN-{gamma} and IL-2 but no IL-4 or transforming growth factor (TGF)-ß in response to syngeneic spleen cells, while CD4–CD8{alpha}{alpha}+ i-IEL produced abundant levels of TGF-ß but no IL-2, IFN-{gamma} or IL-4. CD4+CD8{alpha}{alpha}+ i-IEL proliferated in response to exogenous IL-2 but not to IL-15, while CD4–CD8{alpha}{alpha}+ i-IEL could respond to IL-15 as well as IL-2. These results suggest that a significant fraction of CD4+CD8{alpha}{alpha}+ i-IEL belongs to Th1-type T cells capable of responding to self-MHC class I, while CD4–CD8{alpha}{alpha}+ i-IEL are a unique population with a diversified Vß repertoire that respond to IL-15 in rats.

Keywords: cytokines, mucosa, rodent, T lymphocytes

Transmitting editor: T. Hünig


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