International Immunology, Vol 10, 1251-1259, Copyright © 1998 by Oxford University Press
WR Hein and L Dudler
The usage of lambda and kappa light chain variable region genes in
peripheral fetal lamb B cells was assessed at intervals from 61 to 146 days
of gestation (term = 150 days). Transcripts of 12 distinct Vlambda genes
were identified, eight of which belonged to the previously described
families I or II, while four segments defined a new Vlambda family,
provisionally called family VI. A total of six different Vkappa gene
segments were identified and grouped into four families, while two Jkappa
gene segments were expressed. Southern blot analysis indicated that the
sheep Igkappa locus probably contains approximately 10 Vkappa genes and
suggests that the V gene cluster has been duplicated. Throughout fetal
ontogeny, Vkappa transcripts contained more diverse CDR3 sequences than did
Vlambda transcripts, where the V-J junctions were nearly always invariant
and canonical. There was also a shift in usage of Vkappa genes and in the
pattern of Vkappa-Jkappa gene recombination during fetal ontogeny.
ARTICLES
Diversity of Ig light chain variable region gene expression in fetal lambs
Basel Institute for Immunology, Switzerland.
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