International Immunology, Vol 10, 1049-1056, Copyright © 1998 by Oxford University Press
G Suzuki, Y Nakata, Y Dan, A Uzawa, K Nakagawa, T Saito, K Mita and T Shirasawa
SDF-1 is a member of the CXC chemokines. In contrast to other chemokines
that are induced by inflammation, SDF-1 is constitutively produced by
stromal cells. In order to investigate the physiological roles of SDF-1, we
constructed a fusion protein, SDF-1-Cgamma1, composed from murine
SDF-1alpha and the constant region of human IgG. SDF-1-Cgamma1 stained EL-4
T lymphoma cells and the staining was blocked by rhSDF-1beta. The
expression levels of SDF-1R altered along with the T cell maturation. Most
c-kit+ hematopoietic precursors in fetal liver in gestational day (GD) 14.5
embryo were SDF-1R-, while c- kit+ double-negative (DN) thymocytes in the
embryo were positive for SDF-1R. The receptor expression increased along
with T cell maturation up to double-positive (DP) cell stage.
Interestingly, SDF-1R expression was down-modulated after positive
selection; CD69+CD3hi DP and CD3hi single-positive thymocytes were
SDF-1R-/lo. Northern blot analysis demonstrated that SDF-1 and CXCR4 mRNAs
were abundantly expressed in the thymuses of embryo and adult mice. These
results demonstrate that SDF-1R expression is involved in T cell
development in the thymus, particularly in positive selection.
ARTICLES
Loss of SDF-1 receptor expression during positive selection in the thymus
Division of Radiation Health, National Institute of Radiological Sciences, Chiba, Japan.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Min, A. Panoskaltsis-Mortari, M. Kuro-o, G. A. Hollander, B. R. Blazar, and K. I. Weinberg Sustained thymopoiesis and improvement in functional immunity induced by exogenous KGF administration in murine models of aging Blood, March 15, 2007; 109(6): 2529 - 2537. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. B. Gurney and C. H. Uittenbogaart Human Immunodeficiency Virus Persistence and Production in T-Cell Development Clin. Vaccine Immunol., November 1, 2006; 13(11): 1237 - 1245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Witt and E. A. Robey The Ins and Outs of CCR7 in the Thymus J. Exp. Med., August 16, 2004; 200(4): 405 - 409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Yopp, S. Fu, S. M. Honig, G. J. Randolph, Y. Ding, N. R. Krieger, and J. S. Bromberg FTY720-Enhanced T Cell Homing Is Dependent on CCR2, CCR5, CCR7, and CXCR4: Evidence for Distinct Chemokine Compartments J. Immunol., July 15, 2004; 173(2): 855 - 865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. N. Kremer, T. D. Humphreys, A. Kumar, N.-X. Qian, and K. E. Hedin Distinct Role of ZAP-70 and Src Homology 2 Domain-Containing Leukocyte Protein of 76 kDa in the Prolonged Activation of Extracellular Signal-Regulated Protein Kinase by the Stromal Cell-Derived Factor-1{alpha}/CXCL12 Chemokine J. Immunol., July 1, 2003; 171(1): 360 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zaitseva, T. Kawamura, R. Loomis, H. Goldstein, A. Blauvelt, and H. Golding Stromal-Derived Factor 1 Expression in the Human Thymus J. Immunol., March 15, 2002; 168(6): 2609 - 2617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Hargreaves, P. L. Hyman, T. T. Lu, V. N. Ngo, A. Bidgol, G. Suzuki, Y.-R. Zou, D. R. Littman, and J. G. Cyster A Coordinated Change in Chemokine Responsiveness Guides Plasma Cell Movements J. Exp. Med., July 2, 2001; 194(1): 45 - 56. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Nagase, M. Miyamasu, M. Yamaguchi, T. Fujisawa, K. Ohta, K. Yamamoto, Y. Morita, and K. Hirai Expression of CXCR4 in Eosinophils: Functional Analyses and Cytokine-Mediated Regulation J. Immunol., June 1, 2000; 164(11): 5935 - 5943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Suzuki, H. Sawa, Y. Kobayashi, Y. Nakata, K.-i. Nakagawa, A. Uzawa, H. Sakiyama, S. Kakinuma, K. Iwabuchi, and K. Nagashima Pertussis Toxin-Sensitive Signal Controls the Trafficking of Thymocytes Across the Corticomedullary Junction in the Thymus J. Immunol., May 15, 1999; 162(10): 5981 - 5985. [Abstract] [Full Text] [PDF]
|
|