International Immunology, Vol 10, 923-933, Copyright © 1998 by Oxford University Press
S Fuller-Espie, P Hoffman Towler, DL Wiest, I Tietjen and LM Spain
Mutagenic analyses have identified structural motifs important for TCR-
mediated signaling in the antigen-binding chains, CD3 and zeta subunits of
the TCR complex. In this study, we altered selected residues in the
transmembrane and extracellular constant regions of the TCR beta chain and
expressed the mutants in a T hybridoma line bearing endogenous receptor. We
measured cytokine production and apoptosis in response to antigen or
antibody. We found that mutation of one or both of the transmembrane
tyrosine residues in the TCR beta chain caused a marked reduction in
responsiveness. Mutation of the transmembrane serine to alanine also
reduced responses, although less markedly. Immunoprecipitation analyses
showed that the TCR beta mutations did not alter association with zeta.
These experiments identify a signaling role for the transmembrane domain of
the TCR beta chain.
ARTICLES
Transmembrane polar residues of TCR beta chain are required for signal transduction
The Wistar Institute, Philadelphia, PA 19104, USA.
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