Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Umehara, H.
Right arrow Articles by Domae, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Umehara, H.
Right arrow Articles by Domae, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol 10, 833-845, Copyright © 1998 by Oxford University Press

ARTICLES

Co-stimulation of T cells with CD2 augments TCR-CD3-mediated activation of protein tyrosine kinase p72syk, resulting in increased tyrosine phosphorylation of adapter proteins, Shc and Cbl

H Umehara, JY Huang, T Kono, FH Tabassam, T Okazaki, S Gouda, Y Nagano, ET Bloom and N Domae
Department of Medicine, Osaka Dental University, Japan.

Complete T cell activation requires not only the first signal via TCR- CD3 engagement, but also a co-stimulatory signal through accessory receptors such as CD2, LFA-1 and CD28. However, the pathway of co- stimulatory signaling through accessory receptors is incompletely understood. We report here that CD2 provides a co-stimulus for activation of CD3-mediated syk/ZAP-70 family kinase, p72Syk (Syk), in Jurkat T cells. Although cross-linking of CD2 alone or any combination of CD2 with LFA-1alpha, LFA-1beta or CD28 did not induce tyrosine phosphorylation of Syk, co-cross-linking of CD2 with CD3 enhanced CD3- mediated tyrosine phosphorylation of Syk. Enhancement of tyrosine phosphorylation of Syk by CD2 co-stimulation was CD2 antibody concentration-dependent, and time course studies showed that CD2 co- stimulation enhanced Syk tyrosine phosphorylation by 30 s and through 5 min stimulation compared with the control. In vitro kinase assay revealed that co-cross-linking of CD2 with CD3 augmented Syk kinase activity using myelin basic protein as a substrate. Furthermore, CD2 co- stimulation with CD3 resulted in enhanced tyrosine phosphorylation of adapter proteins, such as Shc and Cbl, in an antibody concentration- dependent manner. Finally, CD2 provided a co-stimulatory signals for synthesis of IL-2 in Jurkat cells and phytohemagglutinin (PHA)- activated T cells and for proliferation of PHA-activated T cells. Taken together, these results indicate that CD2 is an important co- stimulatory receptor for CD3-mediated T cell activation and functions in concert with CD3.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Int ImmunolHome page
Z.-X. Jin, C.-R. Huang, L. Dong, S. Goda, T. Kawanami, T. Sawaki, T. Sakai, X.-P. Tong, Y. Masaki, T. Fukushima, et al.
Impaired TCR signaling through dysfunction of lipid rafts in sphingomyelin synthase 1 (SMS1)-knockdown T cells
Int. Immunol., November 1, 2008; 20(11): 1427 - 1437.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
H. Yoshifuji, H. Umehara, H. Maruyama, M. Itoh, M. Tanaka, D. Kawabata, T. Fujii, and T. Mimori
Amelioration of experimental arthritis by a calpain-inhibitory compound: regulation of cytokine production by E-64-d in vivo and in vitro
Int. Immunol., October 1, 2005; 17(10): 1327 - 1336.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
M. Miyaji, Z.-X. Jin, S. Yamaoka, R. Amakawa, S. Fukuhara, S. B. Sato, T. Kobayashi, N. Domae, T. Mimori, E. T. Bloom, et al.
Role of membrane sphingomyelin and ceramide in platform formation for Fas-mediated apoptosis
J. Exp. Med., July 18, 2005; 202(2): 249 - 259.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Zaffran, O. Destaing, A. Roux, S. Ory, T. Nheu, P. Jurdic, C. Rabourdin-Combe, and A. L. Astier
CD46/CD3 Costimulation Induces Morphological Changes of Human T Cells and Activation of Vav, Rac, and Extracellular Signal-Regulated Kinase Mitogen-Activated Protein Kinase
J. Immunol., December 15, 2001; 167(12): 6780 - 6785.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
D. M. A. Bullens, K. Rafiq, L. Charitidou, X. Peng, A. Kasran, P. A. M. Warmerdam, S. W. Van Gool, and J. L. Ceuppens
Effects of co-stimulation by CD58 on human T cell cytokine production: a selective cytokine pattern with induction of high IL-10 production
Int. Immunol., February 1, 2001; 13(2): 181 - 191.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Huang, D. Tilly, A. Altman, K. Sugie, and H. M. Grey
Inaugural Article: T-cell receptor antagonists induce Vav phosphorylation by selective activation of Fyn kinase
PNAS, September 26, 2000; 97(20): 10923 - 10929.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Goda, T. Imai, O. Yoshie, O. Yoneda, H. Inoue, Y. Nagano, T. Okazaki, H. Imai, E. T. Bloom, N. Domae, et al.
CX3C-Chemokine, Fractalkine-Enhanced Adhesion of THP-1 Cells to Endothelial Cells Through Integrin-Dependent and -Independent Mechanisms
J. Immunol., April 15, 2000; 164(8): 4313 - 4320.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.