International Immunology, Vol 10, 749-755, Copyright © 1998 by Oxford University Press
P Kelleher and SC Knight
Dendritic cells (DC) are potent antigen-presenting cells derived from CD34
bone marrow stem cells. They undergo a series of maturational steps that
allow them to stimulate primary T cell responses. Several cytokines are
known to contribute to this process. In this study murine DC maturing from
bone marrow progenitors under the influence of granulocyte macrophage
colony stimulating factor and tumour necrosis factor-alpha were found to
produce IL-12 as measured by ELISA and by flow cytometry to detect
intracellular cytokine. Administration of additional IL-12 from day 3 to 7
of culture altered the function and phenotype of DC; enhanced stimulation
of T cell proliferation by DC in allogeneic mixed leukocyte reactions was
associated with an increase in the surface expression of CD80 on DC. These
effects were dose dependent, and were consistently seen with IL-12 at 25
ng/ml and were less marked with IL-12 at 50 ng/ml. These results show that
IL-12 is both produced by DC and can increase their stimulatory capacity.
The findings suggest that there may be an autocrine effect of IL-12 on DC
maturation and function.
ARTICLES
IL-12 increases CD80 expression and the stimulatory capacity of bone marrow-derived dendritic cells
Antigen Presentation Research Group, Imperial College School of Medicine, Northwick Park Institute for Medical Research, Harrow, Middlesex, UK.
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