International Immunology, Vol 10, 1923-1930, Copyright © 1998 by Oxford University Press
K Kerekes, J Prechl, Z Bajtay, M Jozsi and A Erdei
Murine cells of the B lymphoblastoid line A20 and concanavalin A- elicited
peritoneal macrophages are shown to activate and fix C3 fragments
covalently when incubated in fresh, autologous serum under conditions
allowing the initiation of the alternative complement pathway. For the
detection of cell-bound C3, cytofluorimetry was performed using
FITC-labeled F(ab')2 fragments of anti-mouse C3. Cell- bound C3 fragments
are not internalized or shed by the cells under culture conditions for at
least two hours. When the antigen-presenting capacity of serum-treated
cells was tested using various antigens and experimental systems,
augmentation of the proliferation of antigen- specific T cells was found.
This enhancing effect was particularly pronounced at suboptimal antigen
doses. The elevation of T cell proliferation induced by C3-opsonized
antigen-presenting cells (APC) could be abrogated by F(ab')2 fragments of
goat anti-mouse C3, suggesting the involvement of C3 receptors expressed by
T cells in the process. Using the 7G6 mAb recognizing murine CR1/CR2, the
presence of these complement receptors on activated T cells is demonstrated
by cytofluorimetry and immunoprecipitation, as well. These results point to
the role of C3 bound to acceptor sites on APC in the facilitation of
antigen presentation, providing a further link between innate and adaptive
immunity.
ARTICLES
A further link between innate and adaptive immunity: C3 deposition on antigen-presenting cells enhances the proliferation of antigen-specific T cells
Department of Immunology, Eotvos Lorand University, God, Hungary.
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