International Immunology, Vol 10, 1657-1664, Copyright © 1998 by Oxford University Press
E Medina, SR Talay, GS Chhatwal and CA Guzman
We have previously shown that fibronectin-binding protein I (SfbI) of
Streptococcus pyogenes can act as an adjuvant for mucosal-delivered
antigens (Medina, E., Talay, S. R., Chhatwal, G. S. and Guzman, C. A. 1998.
Eur. J. Immunol. 28:1069). To characterize the underlying mechanism of the
adjuvancity, we investigated the in vitro stimulating activity of SfbI. The
SfbI protein promoted a dose-dependent proliferation of mouse spleen cells.
Studies performed using cellular subpopulations showed that proliferation
involved B cells and was T cell- and macrophage-independent. SfbI also
induced lg production by B cells in a T cell-independent manner. The
kinetics of lg isotype accumulation in supernatant fluids and the analysis
of Ig-secreting cells suggested that SfbI stimulates B cells expressing
different Ig isotypes rather than promoting the isotype switching of single
subpopulations. Experiments performed with recombinant proteins
encompassing different functional domains of SfbI showed that the
fibronectin-binding repeats were responsible for B cell activation. The
sera from mice immunized by the intranasal route with SfbI did not react
with either double-stranded DNA, cardiolipin or collagen. Interestingly,
stimulation with Sfbl also resulted in the up-regulation of MHC class 11
molecules expression by B cells and macrophages. The elucidation of the
underlying molecular events to the immunomodulatory effect exerted by SfbI
will facilitate the exploitation of the potential of this molecule for the
generation of mucosal vaccines.
ARTICLES
Fibronectin-binding protein I of Streptococcus pyogenes promotes T cell- independent proliferation of murine B lymphocytes and enhances the expression of MHC class II molecules on antigen-presenting cells [In Process Citation]
Department of Microbial Pahtogenicity and Vaccine Research, GBF- National Research Centre for Biotechnology, Braunschweig, Germany.
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